EIA

With our LUCIO ® homogeneous enzyme immunoassays (EIA), we have 32 certified to CE assays for the detection of drugs and their metabolites in urine or saliva. LUCIO® Drug EIA Saliva For the detection of different drugs in saliva parameters we offer LUCIO ®-Oral Fluid Drug EIA kits for the 15 most common parameters. LUCIO® Drug EIA Urine For drug analysis in urine, our range of reagents have calibrators and controls for the detection of 17 different analytes. According to the requirements of forensic /toxicology laboratories, we offer calibrators in four concentrations (low, cut-off, intermediate and high) and two additional controls. In addition, some of our calibrators and controls are also available as multi-calibrator. EIA Principle: EIA principle: The LUCIO ® - EIAs are homogeneous enzyme immunoassays of ready-to-use liquid reagents. The assay is based on a competitive reaction between the drug in the sample and the enzyme glucose-6 -phosphate dehydrogenase (G6PDH) to the fixed number of the binding sites of the specific antibody. In the absence of the free drug in the sample, the drug labeled with the drug G6PDH is bound by the specific antibody and the enzyme activity is inhibited. This property allows a relationship between the concentration of drug in the urine and the enzyme activity. G6PDH activity is determined by the conversion of NAD + to NADH spectrophotometrically at 340 nm Automation: LUCIO ®-Drug EIA kits are suitable for use in all common laboratory machines., We can assist you with the planning of the automation in the technical implementation as well as in the validation and preparation of analytical protocols for our EIAs.